OGA activity can determin using a fluorogenic substrate 4-methylumlliferyl-N-acetyl-β-d-glucosaminide (4MU-GlcNAc) (Sigma M9659). 4-Methylumlliferyl (4MU) has excitation and emission wavelengths of 360 and 450 nm, respectively. When conjugat to GlcNAc (4MU-GlcNAc), fluorescence is quench. After removal of GlcNAc by OGA, 4MU fluorescence can quantifi and the results can correct for the […]
